Outline

Introduction (Figure 26.1)

DNA as the Template for RNA Synthesis

> The Predicted Existence of Messenger RNA (Figure 26.2)

> T2 Bacteriophage and the Demonstration of Messenger RNA (Figure 26.3)

> RNA Dynamics in Uninfected Cells

Enzymology of RNA Synthesis: RNA Polymerase (Item #1, p. 960, #2)

> Biological Role of RNA Polymerase (Figure 26.4)

> Structure of RNA Polymerase (Table 26.1)

Mechanism of Transcription

> Initiation of Transcription: Interactions with Promoters (Figure 26.6)

> Initiation and Elongation: Incorporation of Ribonucleotides (Figure 26.8)

> Punctuation of Transcription: Promoter Recognition (Figure 26.11, Figure 26.12, Figure 26.14)

> Punctuation of Transcription: Termination (Figure 26.15)

> > Factor-Dependent Termination (Figure 26.16)

Regulation of Transcription

> The Lactose Operon: Early Evidence for Transcriptional Control of Gene Expression

> > Regulation in the Lactose Operon (Figure 26.17, Figure 26.18)

> > Isolation and Properties of Repressor

> > The Repressor Binding Site (Figure 26.10, Figure 26.19)

> > Regulation of the lac Operon by Glucose: A Positive Control System (Figure 26.21)

> > The CRP-DNA Complex

> Bacteriophage : Multiple Operators, Dual Repressors, and Models for DNA Binding Specificity (Figure 26.23)

> > Genes and Mutations in the System (Table 26.2)

> > The cI repressor and its Operators (Figure 26.24, Figure 26.26)

> > Early Genes in Phage

> > Interactions Between the Two Repressors (Figure 26.27)

> Structure of Cro and cI Repressors and Related DNA-Binding Proteins (Figure 26.28, Figure 26.29, Figure 26.30, Figure 26.31)

> The SOS Regulon: Activation of Multiple Operons by a Common Set of Environmental Signals (Figure 26.32)

> Biosynthetic Operons: Ligand-Activated Repressors and Attenuation (Figure 21.14, Figure 26.33, Figure 26.35, Figure 26.36, Figure 26.15)

> Other Forms of Regulation

> > The Galactose Operon (Figure 26.38)

> > The Arabinose Operon (Figure 26.39)

> > Control by Antisense RNA (Figure 26.40)

> > The Stringent Response

Posttranscriptional Processing

> mRNA Turnover

> Posttranscriptional Processing in the Synthesis of rRNA and tRNA

> > rRNA Processing (Figure 26.41)

> > tRNA Processing (Figure 26.42)