Outline

Introduction (Figure 25.1)

DNA Methylation (Figure 25.3)

Restriction and Modification

> Biology of Restriction and Modification (Figure 25.5, Figure 25.6)

> Properties of Restriction and Modification Enzymes (Table 25.1)

> > Type I

> > Type II (Table 25.2, Figure 25.7)

> > Type III

DNA Repair

> Types and Consequences of DNA Damage

> Biologically Significant DNA Photoproducts: Pyrimidine Dimers (Figure 25.9)

> Direct Repair of Damaged DNA Bases: Photoreactivation and Alkyltransferases

> > Photoreactivation (Figure 25.10)

> > O6-Alkylguanine Alkyltransferase (Figure 25.11)

> > Nucleotide Excision Repair: Excinucleases (Figure 25.12, Figure 25.13)

> Base Excision Repair: DNA-N-Glycosylases (Figure 25.14)

> Postreplication Repair: Recombinational Repair and the SOS Response (Item #1, p. 919)

> > Recombinational Repair (Figure 25.15)

> > Error-Prone Repair and the SOS Response

> Mismatch Repair (Figure 25.16)

Recombination

> Classification of the Recombination Processes (Figure 25.17, Table 25.3)

> Homologous Recombination

> > Breaking and Joining of Chromosomes (Figure 25.18, Figure 4.14, Figure 25.19)

> > Models for Recombination (Figure 25.20, Figure 25.22)

> > Proteins Involved in Homologous Recombination (Figure 25.23, Figure 25.24, Figure 25.28, Figure 25.29, Figure 25.30)

> Site-Specific Recombination (Figure 25.31)

Gene Rearrangements

> Immunoglobulin Synthesis: Generating Antibody Diversity (Figure 25.32, Figure 25.33)

> Transposable Genetic Elements (Figure 25.34, Figure 25.35, Table 25.4, Figure 25.36, Figure 25.37)

> Retroviruses (Figure 25.38, Figure 25.35, Figure 25.39)

Gene Amplification (Figure 25.41)