Eadie Hofstee Plot

The Eadie-Hofstee plot (Figure 11.17) is a way of plotting kinetic enzyme data so as to yield a straight line for reactions obeying Michaelis-Menten kinetics. This is done by plotting reaction velocity (V) versus velocity/substrate concentration (V/[S]). The slope of the line is equal to -K M and the x-intercept is V max.

An advantage of an Eadie-Hofstee plot over a Lineweaver Burk plot (Figure 11.16) (which plots 1/V versus 1/S) is that the Eadie-Hofstee plot does not require a long extrapolation to calculate K M.

See also: Michaelis-Menten Kinetics, Lineweaver-Burk Plot, K M, k cat, and k cat/K M, Analysis of Kinetic Data

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